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鏈霉素(Strep)ELISA檢測(cè)試劑盒（檢雞肉組織/牛奶/蜂蜜，靈敏度0.1ppb,兩步法）
鏈霉素(Strep)ELISA快速檢測(cè)試劑盒
一、原理
本試劑盒采用間接競(jìng)爭(zhēng)ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物鏈霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競(jìng)爭(zhēng)抗鏈霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物鏈霉素的含量成負(fù)相關(guān)，與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物鏈霉素的含量。
二、試劑盒特性
u 試劑盒靈敏度： 0.1ppb
u 孵育溫度 ： 37℃
u 孵育時(shí)間 ： 30min～30min～15min
批內(nèi)、批間差： <10%
u 樣本檢測(cè)下限
雞 肉 ······································ 1 ppb
雞肝、牛奶 ······································ 4 ppb
蜂 蜜/蜂王漿 ······································ 2 ppb
u 交叉反應(yīng)率
鏈霉素 ············································· 100%
慶大霉素 ············································ ﹤0.1%
雙氫鏈霉素 ··········································· 108%
卡那霉素 ············································ ﹤0.1%
u 樣本回收率
牛 奶 ············································ 85±22%
雞肉組織 ············································ 80±22%
蜂蜜/蜂王漿 ·········································· 75±22%
三、試劑盒組成
1 | 微量測(cè)試孔 | 每條8孔，一板12條 | |
2 | 標(biāo)準(zhǔn)液×6瓶 （1ml/瓶） | 0ppb | 0.1ppb |
0.4ppb | 1.6ppb | ||
6.4ppb | 25.6ppb | ||
3 | 酶標(biāo)記物 | 12ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍(lán)色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 10X濃縮復(fù)溶液 | 50ml | 透明帽 |
四、樣品前處理步驟及檢測(cè)分析步驟
詳見產(chǎn)品說明書。
五、儲(chǔ)藏條件和保質(zhì)期
1、 儲(chǔ)藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見包裝盒。
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